The synthesis of silver phyto nanoparticles and their antibiogram were included in this study. This is the first attempt of introducing silver herbal nanoparticle isolation and antimicrobial assessment. It also comprises about the Nanotechnology and their achievements in silver nanoparticles descriptions and production. The herbal leaves and their medicinal properties were already discussed in varieties of ayurvedic studies. The herbal leaves like Ocimum sanctum and Vitex negundo were included to analyze the productivity of nanoparticles. The synthesis of silver phyto nanoparticles were prepared by adding silver nitrate solution (10-3 M) and the silver phyto nanoparticles were isolated from these herbal leaves. The silver phyto nanoparticles were collected from each herbal plant and tested their antibacterial activity. The test cultures included in this study were Staphylococcus aureus, Vibrio cholerae, Proteus vulgaris and Pseudomonas aeruginosa. The pellets obtained were diluted at the concentration of 1:10 and were taken as 25μl, 50μl, 100μl and 150μl respectively are included in this study. The antibacterial activities of all the herbal nanoparticles obtained from Ocimum sanctum showed maximum inhibitory rate using 150μg of these plants extract compared with Vitex negundo. The silver nanoparticles from herbal leaves showed a good antibacterial activity than the plants used. Further studies needed to find out the efficacy, longevity and toxicity to improve the current investigation.